Доктор Гук та його колеги опублікували свої висновки про позитивну роль використання простагландину Е1, природного лікарського простагландину, у зменшенні травм, які зазвичай спостерігаються при станах, що знижують кишковий кровотік.

Для перевірки впливу простагландину Е1 на 2,5 години ішемії з наступною 2 годиною реперфузії безперервні вимірювання оксиду азоту (електрохімічні) корелювали з періодичними аналізами рівнів супероксиду і пероксинітриту (хемілюмінесценція) та ішемічним/реперфузійним пошкодженням у привідному м’язі кроля. Administering prostaglandin E₁ (1 μg/kg) before or during ischemia/reperfusion caused normalization of the release of nitric oxide, superoxide, and peroxynitrite to slightly above preischemic levels. This pattern was dramatically different from that observed during ischemia/reperfusion alone, where nitric oxide concentration increased three times above its basal level. Normalization of constitutive nitric oxide synthase activity in the presence of prostaglandin E₁ was associated with a significant reduction of superoxide and peroxynitrite production and subsequent reduction of ischemia/reperfusion injury. At 2 h of reperfusion, vasoconstriction associated with ischemia/reperfusion injury was eliminated, and edema was significantly mollified but still apparent. Prostaglandin E₁ treatment does not directly inhibit constitutive nitric oxide synthase, like the inhibitor N^w-monomethyl-L-arginine. Some phenomenon associated with ischemia turns on endothelial constitutive nitric oxide synthase to start transforming L-arginine and oxygen into nitric oxide, but prostaglandin E₁ seems to inhibit this phenomenon. Thus, essential local L-arginine pools are not depleted, and normal basal levels of essential nitric oxide are maintained, whereas cytotoxic superoxide and peroxynitrite production by L-arginine-deficient constitutive nitric oxide synthase is prevented.

(I Huk 1, V Brovkovych, J Nanobashvili, C Neumayer, P Polterauer, M Prager, S Patton, T Malinski Affiliations expand PMID: 10947172 DOI: 10.1097/00024382-200014020-00026)

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Editorial comment

NITRIC OXIDE SYNTHASE AND TISSUE INJURY

Traber, Daniel L. Author Information Shock: August 2000 – Volume 14 – Issue 2 – p 243-244

The “Shock” publication that featured Dr Huk and his colleagues’ work on nitric oxide also included the worthy praise of the editor Daniel L. Traber, whose editorial comment focused on Dr Huk’s work, further demonstrating the importance of these findings. The full comment and article can be read here link.